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The authors claimed the size change could promote the passive targeting via leaking vasculature (7–150 nm) and meanwhile prevent homogenous leakage of SPIONs into normal tissues

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Myeloperoxidase (MPO) activity, a marker of neutrophil infiltration, was evaluated by monitoring the H₂O₂-dependent oxidation of tetramethylbenzidine (TMB) and measuring absorbance at 650 nm