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Standard injection protocol: Dose: 1 to 2 mg daily Duration: 10 to 20 day cycles Frequency: 1 to 3 cycles per year, spaced 4 to 6 months apart Timing: Evening administration to align with natural melatonin production timing Administration: Subcutaneous or intramuscular Maintenance protocol (sublingual): Daily sublingual sprays between injection cycles Lower bioavailability than injection but convenient for ongoing support The cyclic nature of Epitalon protocols reflects how the peptide works

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As a final consideration in defining the causative factors that contribute to increased OS in maternally aged oocytes, it is notable that the oxidative defence capacity of surrounding granulosa and cumulus cells have also been reported to decline with age 102,103,104